The proposed rules revise several definitions used in Chapter 62-302, F.A.C., by updating reference information as well as updating a number of biological standard operating procedures that are incorporated by reference. The proposed rules change the classification of a number of estuarine waters around the state from Class III to Class II (shellfish propagation or harvesting) waters, with specific areas identified by incorporation of maps by reference. The water quality criteria for alkalinity are revised to address waterbodies with naturally low levels of alkalinity. The water quality criteria for un-ionized ammonia in fresh water are replaced with criteria for total ammonia. The water quality criteria for bacteriological quality (fecal coliform) are replaced with criteria for Escherichia coli bacteria (fresh water) and Enterococci bacteria (marine waters), although Class II waters still also retain fecal coliform criteria as well. New water quality criteria are established for nonylphenol, carbaryl (fresh water only), chlorpyrifos, and diazinon. The proposed rules clarify the methodology for determining lake color and alkalinity when assessing nutrients in lakes. The estuarine nutrient region boundaries for Naples Bay and Tampa Bay are slightly revised. Clarification is made to the total nitrogen methodology for Sarasota Bay. The proposed rules also clarify how to calculate annual geometric and arithmetic means for nutrients in marine waters. The proposed rules also update several rule references.